Pipette operation flow

How to use the pipette :

1 adjustment range

When adjusting the range, if you want to adjust from a large volume to a small volume, follow the normal adjustment method, turn the knob counterclockwise; but if you want to adjust from a small volume to a large volume, you can first rotate the scale knob clockwise to over The scale of the range is then adjusted back to the set volume, which guarantees the highest accuracy of the measurement. During this process, never push the button out of the range, otherwise it will get stuck in the internal mechanism and damage the pipette.

2 assembly gun head (suction nozzle)

When placing the pipette on the pipette, the correct method is to insert the pipette (gun) vertically into the pipette tip and slightly tighten it to make it tightly coupled. If it is a multi-channel (such as 8- or 12-channel) pipette, you can align the first line of the pipette with the first head, then insert it obliquely, and shake it in the front-rear direction. The logo of the gun head is slightly over the O-ring and the connecting part can be seen to form a clear seal.

3 pipetting method

Before pipetting, ensure that the pipette, tip, and liquid are at the same temperature. When pipetting the liquid, the pipette is held upright and the tip is inserted 2-3 mm below the surface. Before aspirating, you can first aspirate the liquid several times to wet the liquid suction nozzle (especially when you want to absorb a liquid that is thick or different in density from water). Two pipetting methods can be used at this time.

The first is the forward pipetting method. Press the button to the first stop with your thumb, then slowly release the button to return to the origin. Then press the button to the first stop to discharge the liquid, and then stop for a while and continue to press the button to the second stop to blow out the residual liquid. Finally release the button.

The second is the reverse pipetting method. This method is generally used to transfer highly viscous liquids, biologically active liquids, easily foaming liquids or very small amounts of liquids. The principle is to inhale more liquid than the set range first, and it is not necessary to blow out the residual liquid when transferring the liquid. Press the button first to the second stop and slowly release the button to the origin. Then press the button to the first stop point to discharge the liquid with the set range, continue to hold the hold button at the first stop point (do not press down again), remove the gun head with residual liquid, and discard it.

4 correct placement of the pipette

After use, you can hang it vertically on the pipette holder, but be careful not to fall off. When there is liquid in the pipette tip, do not place the pipette horizontally or upside down to prevent the liquid from flowing back to corrode the piston spring.

The above is a brief introduction to the “pipette operation procedure”. For more information, please read: pipette

In 2007, Shanghai Bayu ( ) Industrial Co., Ltd. established an offline supply system for laboratory instruments, MRO industrial products, etc. In 2009, it officially launched an online procurement operation platform.

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